ĐÁNH GIÁ ĐA DẠNG DI TRUYỀN CỦA GIỐNG LAN RƢ̀ NG Dendrobium THU THẬP TẠI THÀNH PHỐ HỒ CHÍ MINH VÀ RƢ̀ NG NAM CÁT TIÊN BẰNG KỸ THUÂṬ RAPD

LINK DOWNLOAD MIỄN PHÍ TÀI LIỆU "ĐÁNH GIÁ ĐA DẠNG DI TRUYỀN CỦA GIỐNG LAN RƢ̀ NG Dendrobium THU THẬP TẠI THÀNH PHỐ HỒ CHÍ MINH VÀ RƢ̀ NG NAM CÁT TIÊN BẰNG KỸ THUÂṬ RAPD": http://123doc.vn/document/554259-danh-gia-da-dang-di-truyen-cua-giong-lan-r-ng-dendrobium-thu-tha-p-tai-tha-nh-pho-ho-chi-minh-va-r-ng-nam-cat-tien-bang-ky-thuat-rapd.htm

v
SUMMARY

Genetic diversity analysis of wild Dendrobium stem at Ho Chi Minh city
and Nam Cat Tien forest by RAPD
Supervisor: Tran Thi Dung
1
, PhD; Vo Thai Dan
2
, PhD; Luu Phuc Loi
1
, BSc
Student: Truong Minh Dung
1
1
Department of Biotechnology, Nong Lam university, Ho Chi Minh city
2
Faculty of Agriculture, Nong Lam university, Ho Chi Minh city
To analysis the genetic diversity of wild Dendrobium orchid, six decamers were
used for 10 wild Dendrobium species, collected from Ho Chi Minh city and Nam
Cat Tien forest (Dong Nai province). Collecting 10 species from Ho Chi Minh city
and Nam Cat Tien forest (Dong Nai province), optimizing of protocols for DNA
extraction and purification, constructing of protocol for RAPD, analyzing the
genetic of 10 wild Dendrobium species by RAPD and constructing the dendrogram
by NTSYSpc 2.1 software, Winboot program are the objective of the study.
Methods
- To identify 10 wild Dendrobium species with genotype.
- Extraction of total DNA from fresh tissue of 10 wild Dendrobium species.
- Six random primer: OPAC10, OPAH13, OPB01, OPB08, S1384, U693 were
used to analysis of genetic diversity.
- Following RAPD reactions, dendrogram were constructed by NTSYSpc 2.1
and Winboot program.
Conclusion
Six decamers are enough to compare the identity of all samples. We could use
those primers to establish the methodology to perform genetic comparisons in
Vietnam. Besides, the genetic diversity among 10 wild Dendrobium species were
established to support the breeder overcome difficulties in plant salection.


vi


 Trang






iii
  iv
SUMMARY v
 vi








 ix






 x








x






 xi

1. 



 1
1.1.  2
1.2.  

ung  

 2
2. 





 3
Dendrobium 3
 3
Dendrobium 5
 6
  8
 11
 11
 14
 14
2.3.2.2. Primer 14
2.3.2.3. dNTP 16
2.3.2.4. MgCl2 17
2.3.2.5. Taq 17
vii
2.3.5.6. Buffer 17
 
 18
 18
 18
 18
 19
  19
2.5. 









Winboot 26
2.5.1. NTSYSpc version 2.1 26
2.5.2. Winboot 28
2.6 29
2.6.1.  29
2.6.2. nh h 31
3. 











 32
 32
 32
3.1.2  32
 32
Dendrobium  32
3.2.2. T-  34
 34
 36
 36
 36
-  45
4.1. 







Dendrobium 



 


















 45

viii
4.2.  






















 48
4.3. 














 53
4.4. 







10 

Dendrobium
d  54
4.5. 







10 

Dendrobium
b 2.1 Winboot 60


-  69
5.1. 

 69
5.2. 

70
 71

















ix














AFLP: Amplified Fragment Length Polymorphism
bp: base pair
CTAB: Cetyl Trimethyl Ammonium Bromide
DNA: Deoxyribonucleic acid
dNTP: Deoxyribonucleotide triphosphate
EB: extraction buffer
EDTA: Ethylene Diamine Tetra acetic Acid
mM: milimolar (milimol/lite)
NCT: 


OD: Optical density
PCR: Polymerase Chain Reaction
pmol: Picomol
RAPD: Random Amplified Polymorphic DNA
RFLP: Restriction Fragment Length Polymorphism
RNA: Ribonucleic acid
Rnase: Ribonuclease
SSR: Simple Sequence Repeat
Ta : Annealing t
TAE: Tris  Acetate  EDTA
TE: Tris  EDTA

TP. HCM: 

UPGMA: Unweighted Pair Group Method with Arithmetic
VNTR: variable number tandem repeat
x







 Trang
 11
 34

 37
 40
 42
 42
4.1. 













Dendrobium 46
4.2. 












Dendrobium 47
B





 50
4.4. 

(ng/)  51
 53
 53
 55
Dendrobium thu




 62












 Trang

3 qu 50
 51



xi







 Trang
Dendrobium 5
 13
 21
 23
 25
.  26
2.7. 

2.10m 27
2.8. 



Winboot 29
Dendrobium  33
4.1. 













Dendrobium 45
2 49
3 49
4 50
5. 


Dendrobium  54
4.6. 
Dendrobium  55
7. 
Dendrobium 

. 56
8. 
Dendrobium h. 56
9. 
Dendrobium 

. 57
. 
 Dendrobium  57
11. 
 Dendrobium 

. 58
xii
2. 
Dendrobium 

 

. 58
3. 
Dendrobium 

. 59
4. 
Dendrobium . 59
5. 
 Dendrobium 

. 60
6. 
 Dendrobium  



. 60
7. 
g Dendrobium 

. 61
8. 


n Dendrobium 

Nam
 63
4.19. 












 Dendrobium . 64
4.20. 3












Dendrobium . 65












1







1.1. 
   

 








 T H , Nam
C Tin c nhiu iu kin t nhin th vic ph trin nhiu
    , 





  Dendrobium. Lan Dendrobium 
















 . 

 
 


  

Dendrobium.


 ,  ,
m 

 

 

 Dendrobium 










.  ,              
lan 

Dendrobium 



 
          
lan Dendrobium .
n, 



 
 isozyme hay ch 
(RFLP, AFLP, RAPD, SSR). T v



Dendrobium 

.


2






,  













 : 




ng di truyn ca ging lan rng Dendrobium thu th

  H




 





.








  t
Nam T H 
1.2.   

 y


 Dendrobium, 










.



- 







 Dendrobium 






















.
- 


















.
- 









 .
- 











 Dendrobium 


primer RAPD.
- 














NTSYSpc version 2.1 .

- 

















.
- 



.
-  RAPD , 



.
- Dendrobium 
.
- M Dendrobium 


Winboot.